Biochem/physiol Actions

Target IC₅₀: 1.7 nM, 40 pM, against protein phosphatase 1 (PP1), protein phosphatase 2A (PP2A), respectively

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary Targetprotein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)

General description

Cyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC₅₀ = 1.7 nM) and PP2A (IC₅₀ = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.

Legal Information

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.Amick, G.D., et al. 1992. Biochem. J.287, 1019.Davidson, H.W., et al. 1992. J. Cell Biol.116, 1343.Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.MacKintosh, C., et al. 1990. FEBS Lett.264, 187.Cohen, P., et al. 1989. FEBS Lett.250, 596.Carmichael, W.W. 1988. in Handbook of Natural Toxins3,Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.

Packaging

Packaged under inert gas

250 µg in Plastic Bag(s)

yes

Physical form

A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475815) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Highly Toxic (H)