Analysis Note

Appearance (clearness): clearAppearance (colour): redpH-value (25 °C): 7.2 - 7.6Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very goodGrowth (Citrobacter freundii ATCC 8090): good to very goodGrowth (Enterobacter cloacae ATCC 13047 (WDCM 00083)): good to very goodGrowth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very goodGrowth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very goodGrowth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very goodGrowth (Proteus mirabilis ATCC 14153): good to very goodGrowth (Proteus hauseri ATCC 13315): good to very goodButt (Escherichia coli ATCC 25922 (WDCM 00013)): yellowButt (Citrobacter freundii ATCC 8090): yellow/blackButt (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellowButt (Shigella flexneri ATCC 12022 (WDCM 00126)): yellowButt (Salmonella typhimurium ATCC 14028 (WDCM 00031)): yellow/blackButt (Salmonella enteritidis ATCC 13076 (WDCM 00030)): yellow/blackButt (Proteus mirabilis ATCC 14153): yellow/blackButt (Proteus hauseri ATCC 13315): yellow/blackSlant surface (Escherichia coli ATCC 25922 (WDCM 00013)): yellowSlant surface (Citrobacter freundii ATCC 8090): yellowSlant surface (Enterobacter cloacae ATCC 13047 (WDCM 00083)): yellowSlant surface (Shigella flexneri ATCC 12022 (WDCM 00126)): redSlant surface (Salmonella typhimurium ATCC 14028 (WDCM 00031)): redSlant surface (Salmonella enteritidis ATCC 13076 (WDCM 00030)): redSlant surface (Proteus mirabilis ATCC 14153): red/blackSlant surface (Proteus hauseri ATCC 13315): yellowIncubation: 24 hrs.; 35 °C; aerobic.

Application

GranuCult^® Triple sugar iron agar is suitable for the identification of Enterobacteriaceae in meat, meat products, and milk. It is commonly used as a biochemical test in characterization of a microorganism. Depending on the extent of fermentation and production of gas or production of H₂S, the type of the microorganism can be determined.

Features and Benefits

Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment Excellent wettability, solubility, and free flowing properties Convenient, with minimal component separation and clumping, even under warm or humid conditions High batch-to- batch reproducibility Prolonged shelf life of up to five years High number of test strains exceeding all regulatory demands Granulation technology allowing many supplements to be included, with no need to add these separately

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

GranuCult^® Triple sugar iron agar is recommended for the identification of Gram-negative enterobacteria. It contains lactose, sucrose, and glucose as fermentable carbohydrates. The pH indicator, phenol red, indicates the degradation of sugars to produce acids by the varying color of the medium (from red to yellow). Several species of bacteria reduce thiosulfate to hydrogen sulfide that combines with ferric ions of iron salt to produce iron sulfide, an insoluble black precipitate.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 g in Plastic bottle