Analysis Note

Appearance (colour): slightly yellowAppearance (description): leafsAssay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2K (Potassium): ≤ 0.02 %Na (Sodium): ≤ 0.5 %Mg (Magnesium): ≤ 0.01 Ê (Calcium): ≤ 0.05 %Heavy metals (as lead): ≤ 0.001 úts: ≤ 0.2 Þcomposition products: not detectableWater (according to Karl Fischer): ≤ 5.00 %Ash (600 °C): ≤ 1 %NADH Oxidase (NADH; pH 7.5; 25° C): not detectableLDH (pyruvate; pH 7.5; 25° C): not detectable

Application

Albumin fraction V has been used: as a component of perfusion buffer for perfusion protocol for rodent organ perfusion as a blocking agent in western blot analysisas a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA)is the chief component ofcell culturemedia and also works as ablocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial forembryonic stem cells (hESC) differentiationandassists the transportof drugs, hormones, and fattyacids.

General description

Bovine serum albumin (BSA) belongs to the serum albumin family withthree domains and two sub-domainseach. This globular, α-helical,non-glycosylated proteinhas 17-disulfidebonds.

Packaging

25, 100 g in Glass bottle