Application

Pseudomonas Isolation Agar is used as a selective and differential medium for primary isolation and identification of Pseudomonas from clinical and nonclinical specimens.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Pseudomonas Isolation Agar is used as a selective and differential medium for primary isolation and identification of Pseudomonas aeruginosa. It is a modified medium based on the formulation of Medium A by King et al. and developed to differentiate Pseudomonas aeruginosa from other Pseudomonas sp. based on pigment formation. Peptic digest of animal tissue provides nitrogenous compounds. Glycerol serves as carbohydrate source and promotes the phycocyanin (blue-green pigment) production. Magnesium is a cofactor for many metabolic reactions and together potassium sulphate stimulates phyocyanin production as well. Irgasan is an antibiotic and selective inhibits gram-positive and gram-negative bacteria other than Pseudomonas spp. Agar is the solidifying agent.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 45.03 g in 1 litre distilled water containing 20 ml glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.