5(6)-Carboxy-2,7-dichlorofluorescein diacetate, BioReagent, suitable for fluorescence, >=85% (HPCE)

Code: 21884-100mg D2-231

Analysis Note

Excmax: 504 nm (Lit.)

Application

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used ...


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Analysis Note

Excmax: 504 nm (Lit.)

Application

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used as a cell permeate probe to detect intracellular reactive oxygen species (ROS). The anionic fluorescent dye, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is suitable for studying Multi-Drug Resistance (MDR) by target cells.

General description

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Other Notes

Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

assay≥85% (HPCE)
fluorescenceλex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)
InChI keyLZMPJBOMBCYYMP-UHFFFAOYSA-N
InChI1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
mp210 °C (dec.) (lit.)
product lineBioReagent
Quality Level100
SMILES stringCC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)cccc(cc9)C(O)=O)c7cc6Cl
solubilitymethanol: soluble, DMSO: soluble, DMF: soluble
storage temp.−20°C
suitabilitysuitable for fluorescence
Cas Number127770-45-0
This product has met the following criteria: