Biochem/physiol Actions

Reversible: no

Primary Targetcaspase-3, caspase-6, caspase-7, caspase-8, caspase-10

Product does not compete with ATP.

Cell permeable: yes

General description

A potent, cell-permeable, and irreversible inhibitor of caspase-3 as well as caspase-6, caspase-7, caspase-8, and caspase-10. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 µg/75 µl) solution of Z-DEVD-FMK (Cat. No. 264156) in DMSO is also available.

A potent, cell-permeable and irreversible inhibitor of CPP-32/apopain, a member of the ICE/CED-3 family of cysteine proteases.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Masuda, Y., et al. 1997. Biochem. Biophys. Res. Commun. 234, 641.Nicholson, D.W. 1996. Nature Biotech. 14, 297.Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.Nicholson, D.W., et al. 1995. Nature 376, 37.

Packaging

1 mg in Plastic ampoule

250 µg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Z-Asp(OCH₃)-Glu(OCH₃)-Val-Asp(OCH₃)-FMK

Warning

Toxicity: Standard Handling (A)