Biochem/physiol Actions
Reversible: no
Primary Targetcaspase-3, caspase-6, caspase-7, caspase-8, caspase-10
Product does not compete with ATP.
Cell permeable: yes
General description
A potent, cell-permeable, and irreversible inhibitor of caspase-3 as well as caspase-6, caspase-7, caspase-8, and caspase-10. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 µg/75 µl) solution of Z-DEVD-FMK (Cat. No. 264156) in DMSO is also available.
A potent, cell-permeable and irreversible inhibitor of CPP-32/apopain, a member of the ICE/CED-3 family of cysteine proteases.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Masuda, Y., et al. 1997. Biochem. Biophys. Res. Commun. 234, 641.Nicholson, D.W. 1996. Nature Biotech. 14, 297.Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.Nicholson, D.W., et al. 1995. Nature 376, 37.
Packaging
1 mg in Plastic ampoule
250 µg in Plastic ampoule
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Sequence
Z-Asp(OCH₃)-Glu(OCH₃)-Val-Asp(OCH₃)-FMK
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria: