Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable forDNA staining in agarose gelsanalysis of changes in DNA during apoptosisdetection of mycoplasmaphotofootprinting of DNAimmunofluorescent staining of cellsDAPI has been used:-in rapid monitoring of microbial contamination in chromosomal banding techniquein detection of apoptotic cellsin fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNAto stain mature pollen grains(0.5 mg/ml)

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

Packaging

1, 5, 10, 50 mg in glass bottle