Application

N-acetyl-β-D-glucosaminidase (NAG) has been used:as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)as a substrate to study chitinase ChiB1 activityto assess degranulation using rat basophilic leukemia (RBL)-2H3 cells

Biochem/physiol Actions

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

Packaging

1 g in poly bottle

100, 250, 500 mg in poly bottle

Substrates

Chromogenic substrate for N-acetyl-glucosaminidase.