Analysis Note

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Application

Trypsin Inhibitor from Glycine max (soybean) has been used:as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestrisas a standard to compare the trypsin inhibitory activity of the purified proteinto monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography as an trypsin inhibitor

Biochem/physiol Actions

Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

General description

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.

Other Notes

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Packaging

5, 10, 25 mg in glass bottle

Preparation Note

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Unit Definition

One trypsin unit will produce a δA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.