Application

cOmplete^™, Mini, EDTA-free protease inhibitor cocktail has been used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.

cOmplete^™Mini, EDTA-free Protease Inhibitor Cocktail has used as a component of resuspension buffer for nuclear lysates preparation from prostate cancer cell lines for chromatin and RNA immunoprecipitation resuspension buffer for protein extraction from neurospheres for western blot analysis homogenization buffer for protein extraction from rat left ventricle samples and immunoprecipitaion buffer for human embryonic kidney 293 (HEK293) cells

Components

Proprietary mixture of several protease inhibitors.

Features and Benefits

Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results. Easy-to-use: Simply drop a quick-dissolving tablet into your buffer. Safe: Choose non-toxic inhibitors that pose no risk to you or those around you. Complete protection: Instantly protect your proteins against a broad range of proteases. Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.

General description

cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets, they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Legal Information

cOmplete is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Tablets in glass vials

Preparation Note

Working concentration: 1 tablet per 10 ml extraction solutionWorking solution: Preparation of Working Solution:One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.Stock solution (7 X conc.):Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Quality

Performance-tested with pancreas extract.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Specificity

For the inhibition of serine, cysteine, but not metalloproteases.