Components

CMV FITC-Labeled Monoclonal Antibody - (Catalog No. 5090). One10 mL dropper vial containing FITC conjugated monoclonal antibody against CMV immediate early antigen IE1 and IE2 (MW 68 to 72 kDa, CMV AD169 derived protein). The monoclonal is an IgG 2a isotype, complete molecule antibody. Conjugate contains protein stabilizer, Evans blue, Tween-20 and 0.1% sodium azide (preservative).CMV Control Slides - (Catalog No. 5027). Two slides containing one well of CMV-infected cells (positive) and one well of uninfected cells (negative).Phosphate Buffered Saline (PBS) - (Catalog No. 5087). One packet of phosphate buffered saline salts.Tween 20/Sodium Azide Solution (100X) - (Catalog No. 5037). One10 mL vial containing Tween 20/sodium azide concentrate.Mounting Fluid - (Catalog No. 5013). One 10 mL dropper vial containing tris-buffered glycerin (pH 9.0 ± 0.5), a fluorescence enhancer and 0.1% sodium azide (preservative).

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 3255

Storage and Stability

When stored at 2-8°C, the CMV kit is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date.

Warnings and Precautions:

* Sodium azide, present in the conjugate, PBS, and mounting fluid, can form potentially explosive metal azides with lead and copper pipes. Upon disposal, flush with large volumes of water to prevent build-up in plumbing.

* Pooling, substitution, or alteration of any reagent may cause erroneous results.

⋅ Do not mix or substitute reagents from different kit lots.

* Do not allow the shell vials or slides to dry at any time during the staining procedure.

* Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite.

* Acetone and methanol are extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

* Avoid contact with Evans blue (present in the Anti-CMV FITC-labeled monoclonal antibody) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

* Avoid contact with the Mounting Fluid, which contains a fluorescence enhancer that may be destructive to the tissue of mucous membranes. If contact occurs, flush with large volumes of water.

* Performance of the fluorescence microscope is of critical importance in achieving satisfactory test results. Microscope objectives, bulb intensity and wattage, and filters may affect results.

* PBS with Tween 20 and/or sodium azide should not be used in viral isolation procedures.

* Do not mouth pipette reagents.