SULFO-SANPAH (SULFOSUCCINIMIDYL 6-(4-A

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Features and Benefits

Reactive groups: sulfo-NHS ester and nitrophenyl azide Reactive towards: amino groups and any nucleophile Non-cleavable N-Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation Water soluble; non-cleavable

General description

ANB-NOS and Sulfo-SANPAH are heterobifunctional crosslinkers that contain an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. When exposed to UV light nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).