Application

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Acrylamide/Bis-acrylamide, 30% solution has been used:in the preparation of polyacrylamide gel for photobleaching experimentas a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solutionin the preparation of polyacrylamide gels during western blot analyses

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.