Amido Black Staining Solution 2X, electrophoresis reagent

Application

Amido Black Staining Solution 2X has been used in the staining:serum proteins present in the culture supernatantcysteine sulfinic acid decarboxylase protein in mouse liver homogenatehistones from chromatin samples

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.