Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.Western Blotting and EIABrowse additional application references in our FLAG® Literature portal.
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.Method of purification - Affinity tag purification
Immunogen
FLAG; peptide sequence DYKDDDDK
Legal Information
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Specificity
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
This product has met the following criteria: