Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution

Code: F1804-5MG D2-231

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immu...


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Your Price
£1,670.00 5MG
£2,004.00 inc. VAT

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.Western Blotting and EIABrowse additional application references in our FLAG® Literature portal.

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcemouse
cloneM2, monoclonal
concentration1 mg/mL
conjugateunconjugated
formbuffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
immunogen sequenceDYKDDDDK
isotypeIgG1
purified byaffinity chromatography (affinity tag purification)
Quality Level200
shipped inwet ice
species reactivityall
storage temp.−20°C
This product has met the following criteria: