Fast Green FCF, certified by the Biological Stain Commission

Code: F7258-25G D2-231

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than ...


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Your Price
£174.00 25G
£208.80 inc. VAT

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Fast Green FCF has been used:along with safranin for the staining of nodule sections in the collagen deposit staining procedure for the staining of protein and starch as a dye to detect and inject DNA solution in chicken embryo

Packaging

25 g in glass bottle

Suitability

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

application(s)diagnostic assay manufacturinghematologyhistology
compositiondye content, ≥90%
formpowder
gradecertified by the Biological Stain Commission
InChI keyRZSYLLSAWYUBPE-UHFFFAOYSA-L
InChI1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
mp290 °C (dec.) (lit.)
Quality Level200
SMILES string[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(C=C3)=[N+](CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(O)cc5S([O-])(=O)=O
solubilityH2O: 1 mg/mL
storage temp.room temp
Cas Number2353-45-9
This product has met the following criteria: