Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

CTTNBP2NL (Cortactin-binding protein 2 N-terminal–like protein) gene encodes a homologue of CTTNBP2 that interacts with cortactin that regulates dendritic spinogenesis in neurons. It is present in stress fibers while CTTNBP2 is located in the cortex and intracellular puncta. In HEK293 cells, the striatin–protein phosphatase 2A (PP2A) protein complex is found to associate with CTTNBP2NL. PP2A complex functions in synaptic signaling and neurodegeneration. The sequence of this protein is similar to the short form of CTTNBP2 called as CTTNBP2-S, especially in the N-terminal region, coiled-coil domain, and C-terminal proline-rich motifs. The proline-rich motif of CTTNBP2NL interacts with cortactin and targets it to stress fibers. It also associates with F-actin bundles.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

Immunogen

CTTNBP2 N-terminal-like protein recombinant protein epitope signature tag (PrEST)

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST71339.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide