Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 µg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in insulin-producing cell (IPC) differentiation, infection inhibition assays, and proliferation assays.

This product is supplied at a concentration of 1 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2µm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

• Biological source: mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
• Sterility: sterile; sterile-filtered
• Product line: BioReagent
• Form: aqueous solution
• Concentration: 1-2 mg/mL in Tris-buffered saline
• Surface coverage: 1-2 µg/cm2
• Suitability: cell culture
• Storage: -20°C
• Gene Information: Mouse ... Lama1 (16772), Lamb1 (16777), Lamc1 (226519), Lamc2( 16782)