Poly-L-lysine hydrobromide, mol wt 30,000-70,000

Code: P2636-500MG D2-231

Analysis Note

Molecular weight based on viscosity and was also assayed by MALLS.

Application

Poly-L-lysine polymers can be used in promoting cell...


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Your Price
£626.00 500MG
£751.20 inc. VAT

Analysis Note

Molecular weight based on viscosity and was also assayed by MALLS.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Packaging

25, 100, 500 mg in poly bottle

1 g in poly bottle

Preparation Note

This product has a molecular weight of 30,000-70,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

colorwhite to faint yellow
formpowder
InChI keyWBSCNDJQPKSPII-KKUMJFAQSA-N
InChI1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
mol wt30,000-70,000
Quality Level300
SMILES stringCl.NCCCCC(N)C(O)=O
storage temp.−20°C
technique(s)cell culture | mammalian: suitable, bioconjugation: suitable
Code
Description
Unit Size
List Price
Qty
P2636-100MG
Unit:100MG
List Price: £222.00
Source:List Price
ADD
P2636-1G
Unit:1G
List Price: £1,080.00
Source:List Price
ADD
P2636-25MG
Unit:25MG
List Price: £65.50
Source:List Price
ADD
Cas Number25988-63-0
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