Application

SSC Buffer 20× Concentrate has been usedas a hybridization buffer on in situ hybridization human-specific repetitive Alu sequenceas a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNAas an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Features and Benefits

Convenient ready-to-use liquid Multi-purpose use in hybridizations

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.