HiTrap NHS-activated HP 1 x 5mL

Code: 17071701 D2-84

HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.

  • Easy coupling of any ligand containing primar...


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    £229.00 EACH
    £274.80 inc. VAT

HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.

  • Easy coupling of any ligand containing primary amine groups.
  • Packed with NHS-Activated Sepharose High Performance resin.
  • Convenient prepacked columns can be used with a syringe, pump, or chromatographic system, such as ÄKTA systems.

NHS activated resin for amine coupling

This NHS-activated resin can be used with any primary amine-containing ligand. The resin reacts with primary amine groups to form stable amide linkages that covalently immobilize antibodies or other proteins for affinity purification. The resin enables simple coupling, carried out at close to neutral pH.

The NHS-activated Sepharose High Performance (HP) resin in these columns is delivered in 100% isopropanol to protect the active groups. It contains ~10µmol NHS groups/mL resin.

HiTrap NHS-Activated HP, 5 x 1mLHiTrap NHS-Activated HP, 1 x 5mL
Bed dims, mm7 x 2516 x 25
Bed height, mm2525
Bed volume, mL15
Column, i.d.716
Flow rate, mL/min﹤4﹤20
ApplicationPartial purification of an IgE-stimulating factor from a human T-cell lineRemoval of BSA from an HIV-2 virus lysatePurification of anti-mouse Fc IgG from sheep anti-serumPartial purification of an IgE-stimulating factor from a human T-cell lineRemoval of BSA from an HIV-2 virus lysatePurification of anti-mouse Fc IgG from sheep anti-serum
Pressure max (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Storage4 to 8°C, 100% isopropanol4 to 8°C, 100% isopropanol
Chromatography techniqueGroup specific affinityGroup specific affinity
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17071601
Unit:EACH
List Price: £264.00
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