HiTrap IEX Selection Kit, Cytiva, 17-6002-33

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Fast and easy selection with seven different ion-exchange ligands on Sepharose^® Fast Flow and Sepharose^® XL, prepacked in 1 mL HiTrap^® columns. Offers a fast, easy and convenient way screen for the optimal media for a given application . Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up. Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

General description

HiTrap^® IEX Selection Kit consists of seven HiTrap^® columns, prepacked with different Sepharose^® Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.Q, SP, DEAE, and CM Sepharose^® Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^® 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose^® XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose^® Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose^® 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

Legal Information

Sepharose is a registered trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))