Protein G Sepharose 4 Fast Flow 25 ml

Code: 17061802 L1-23

Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.

  • High binding capacity: approximately 20mg human IgG per mL resin
  • Broad-spect...


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    £3,088.00 EACH
    £3,705.60 inc. VAT

Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.

  • High binding capacity: approximately 20mg human IgG per mL resin
  • Broad-spectrum: binds to a broad range of IgG species and subclasses
  • Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses
  • No specific albumin binding: recombinant protein G has been genetically engineered to remove the albumin binding region
  • Minimal leaching: optimized coupling method minimizes leaching of recombinant protein G and improves binding capacity for IgG
  • Wide range of formats: including loose resin packs from 5mL to 5L, for manual or automated antibody purification and immunoprecipitation procedures

IgG purification

Protein G Sepharose 4 Fast Flow is a good first choice for general-purpose capture of antibodies at laboratory scale. Compared to protein A-based resins, it binds a broader range of immunoglobulins from eukaryotic species and more classes of IgG.

Key applications include:

  • isolation and purification of IgG from serum, ascites, and tissue culture media
  • monoclonal antibody purification
  • isolation of antibody-ligand immune complexes
  • immunoprecipitation protocols

The recombinant protein G ligand binds strongly to the Fc-region from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on protein G. The ligand is immobilized via a multipoint coupling to the Sepharose 4 Fast Flow agarose matrix to minimize ligand leakage.

Protein G Sepharose 4 Fast Flow resin is part of Cytiva's BioProcess media (resins) range, which ensures security of supply and comprehensive technical and regulatory support.

Protein G Sepharose 4 Fast Flow
BioProcess resinYes
LigandRecombinant protein G lacking albumin-binding region, produced in E. coli
Particle size, d50V~90µm
Matrixcross-linked agarose, 4%, spherical
Ligand Coupling MethodCyanogen bromide activation
Ligand density2mg Protein G/mL medium
pH stability, operational3 - 9
pH stability, CIP2 - 10
Pressure/flow characteristicsPressure/flow characteristics: 150-250cm/h at <0.1MPa in a XK 50/60 column with 5cm diameter and 25cm bed height (at 20?C using buffers with the same viscosity as water)
Storage2 to 8°C, 20% Ethanol
Chemical stabilityStable in aqueous buffers commonly used in Protein G chromatography, 6M guanidine hydrochloride pH 4.7, 20mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20mM Sodium phosphate, 100mM Glycine- phosphoric acid, 6M Urea, 20% ethanol + 2% hibitane digluconate
Certificate of AnalysisYes
Chromatography techniqueAntibody affinity chromatography
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17061801
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List Price: £813.00
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17061805
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List Price: £4,943.00
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