IMAC Sepharose High Performance 100 ml

Code: 17092007 D2-84

IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.

  • Supplied uncharged, for customized metal ion chargi...


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    £1,466.00 EACH
    £1,759.20 inc. VAT

IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.

  • Supplied uncharged, for customized metal ion charging and optimized selectivity.
  • Suitable for optimizing purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
  • High performance purification due to its 34µm bead size.
  • High binding capacity.

Uncharged resin for immobilized metal ion affinity chromatography

IMAC Sepharose High Performance is supplied free of metal ions, allowing a user to customize the charge to the target protein. Select a transition metal ion (e.g., Cu2+, Zn2+, Ni2+, or Co2+) that will bind to the covalently immobilized chelating ligand on the Sepharose.

This resin is commonly used for affinity purification of histidine-tagged proteins. However, it is also suitable for purifying native or untagged recombinant proteins.

IMAC Sepharose High Performance
Flow velocity, cm/h<300
Particle size, d50V~34µm
MatrixHighly cross-linked agarose, 6%
Binding Capacity/mL Chromatography MediumAt least 40mg histidine-tagged protein/mL resin when changed with Ni2+
pH stability, operational3 - 12
pH stability, CIP2 - 14
Storage4 to 30°C, 20% Ethanol
Chemical stabilityStable in commonly used buffers, At 40°C 0.01M HCl, 0.1M NaOH [For One Week] 1M NaOH, 70% acetic acid [For 12h] 30% 2-propanol [30min] 2% SDS [1h]
Avoid UsingChelating agents, e.g. EDTA, EGTA, citrate
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