Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.
- Proven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors.
- Based on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
- Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Applications of heparin affinity chromatography
Cytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.
Chemically stable heparin purification resin
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.
This product has met the following criteria: