HiTrap rProtein A FF 1 x 5mL

Code: 17508001 D2-84

HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and a...


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£952.00 EACH
£1,142.40 inc. VAT

HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Well-established protein A-based resin used in many approved MAb processes.
  • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

HiTrap rProtein A FF, 5 x 1 mlHiTrap rProtein A FF, 2 x 1 mlHiTrap rProtein A FF, 1 x 5 mlHiTrap rProtein A FF, 5 x 5 ml
Bed dimensions, mm7 x 257 x 2516 x 2516 x 25
Bed height, mm25252525
Bed volume, mL1155
Column i.d.771616
Flow rate, mL/min<4<4<20<20
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Storage4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol
Chromatography techniqueAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatography
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17507901
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