Ni Sepharose High Performance nickel-charged IMAC resin is for high-resolution his-tagged protein purification with high binding capacity for maximized recovery.
- High resolution – due to small bead size (average bead size is 34 µm).
- High binding capacity for high protein recovery – exceeds 40 mg histidine-tagged (his-tagged) protein/mL resin.
- Broad reagent compatibility – compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Nickel resin for high recovery of histidine-tagged proteins
Ni Sepharose High Performance (HP) affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
This IMAC resin has a binding capacity that exceeds 40mg/mL, resulting in high protein recovery. The small bead size contributes to a more efficient purification compared to nickel resins of larger bead size.
This product has met the following criteria: