Ni Sepharose High Performance 25 ml

Code: 17526801 D2-84

Ni Sepharose High Performance nickel-charged IMAC resin is for high-resolution his-tagged protein purification with high binding capacity for maximized recovery.

  • High resolution –...


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    £460.00 EACH
    £552.00 inc. VAT

Ni Sepharose High Performance nickel-charged IMAC resin is for high-resolution his-tagged protein purification with high binding capacity for maximized recovery.

  • High resolution – due to small bead size (average bead size is 34 µm).
  • High binding capacity for high protein recovery – exceeds 40 mg histidine-tagged (his-tagged) protein/mL resin.
  • Broad reagent compatibility – compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Nickel resin for high recovery of histidine-tagged proteins

Ni Sepharose High Performance (HP) affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.

This IMAC resin has a binding capacity that exceeds 40mg/mL, resulting in high protein recovery. The small bead size contributes to a more efficient purification compared to nickel resins of larger bead size.

Ni Sepharose High Performance
Particle size, d50V~34µm
MatrixHighly cross-linked agarose, 6%
Binding Capacity/mL Chromatography MediumAt least 40mg histidine-tagged protein/mL resin
Metal Ion Capacityapprox. 15µmol Ni2+/mL medium
pH stability, operational3 - 12
pH stability, CIP2 - 14
Storage4 to 30°C, 20% Ethanol
Chemical stabilityFor Ni2+-stripped medium 0.01M HCl, 0.1M NaOH. Tested for one week at 40°C 1M NaOH, 70% HAc. Tested for 12h 2% SDS. Tested for 1h 30% 2-propanol. Tested for 30min
Avoid UsingChelating agents such as EDTA, EGTA , citrate
Chromatography techniqueHistidine-tagged protein purification
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List Price: £1,570.00
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