GSTrap HP 1 x 5mL

Code: 17528201 D2-84

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

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£387.00 EACH
£464.40 inc. VAT

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

  • High resolution purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

GSTrap HP 1mL and 5mL columns are prepacked with Glutathione Sepharose High Performance resin, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity resin, and impurities are removed by washing with binding buffer

GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.

GSTrap HP 5 x 1mLGSTrap HP 1 x 5mLGSTrap HP 5 x 5mL
Bed dimensions, mm7 x 2516 x 2516 x 25
Bed height, mm252525
Bed volume, mL155
Column i.d.71616
Flow rate, mL/min<4<20<20
ApplicationGlutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Storage4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol
Chromatography techniqueGST-tagged protein purificationGST-tagged protein purificationGST-tagged protein purification
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17528202
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17528101
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