Ni Sepharose 6 Fast Flow 25 ml

Code: 17531801 D2-84

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.

  • In process sc...


    read more

    Your Price
    £417.00 EACH
    £500.40 inc. VAT

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.

  • In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.
  • In research scale applications: This resin binds 40mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.
  • Meets industrial demands with security of supply, robust performance, and regulatory support.
  • Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.
  • Excellent flow properties provide great performance even at high flow rates.

High binding capacity resin with excellent flow properties

Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.

The high dynamic binding capacity combined with the ability to withstand high flow rates gives an overall advantage of being able to increase flow rate and reduce the total time for protein purification considerably with a minimal loss in recovery. Therefore, this immobilized metal affinity chromatography (IMAC) purification resin is a good choice for scaling up.

Ni Sepharose 6 Fast Flow
Particle Size, um45 - 165
BioProcess resinYes
Particle size, d50V~90µm
Matrix6% cross-linked agarose
Binding Capacity/ml Chromatography MediumApprox. 40mg histidine-tagged protein
Metal Ion Capacity~15µmol Ni2+/mL medium
pH stability, operational3 - 12
pH stability, CIP2 - 14
Pressure/Flow SpecificationBase matrix: 250-400cm/h, 100kPa, XK 50/60 column, bed height 25cm
Storage4 to 30°C, 20% Ethanol
Chemical stabilityStable in commonly used aqueous buffers - 0.1M HCl, 0.1M NaOH, 8M urea
Chromatography techniqueHistidine-tagged protein purification
Code
Description
Unit Size
List Price
Qty
17531806
Unit:EACH
List Price: £97.00
Source:List Price
ADD
17531802
Unit:EACH
List Price: £1,461.00
Source:List Price
ADD
17531803
Unit:EACH
List Price: £6,135.00
Source:List Price
ADD
This product has met the following criteria: