Application

Bovine Serum Albumin has been used:to block non-specific binding of the antibodiesas a component of activating medium for inducing sperm motilityto determine the protein concentration of the bacteriocin by the bicinchoninic acid (BCA) method

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

Heat shock fractionated Protease-free

General description

Albumin is abundantly present in human plasma. Increased levels of albumin result in dehydration. Albumin turnover is usually found in children with iron deficiency anemia. It is a predictive indicator for liver disease.

Packaging

1, 5 kg in poly drum

10, 50, 100, 500 g in poly bottle

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.