Amino Acid Sequence

γ-Glu-Cys-Gly

Application

L-Glutathione (GSH) reduced has been used in the elution buffer to elute GST (glutathione S-transferase)-fused proteins using glutathione-agarose beads. It has been used to prepare a standard curve for GSH analyses.

May be used at 5-10 mM to elute glutathione S-transferase (GST) from glutathione agarose.

Biochem/physiol Actions

Reduced L-glutathione (GSH) is a thiol compound and is associated with catabolism and transportation. Plasma GSH levels are associated with Parkinson′s diseases, Alzheimer′s, diabetes, macular degeneration, and HIV (human immunodeficiency virus) disease.

Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.

Packaging

1, 5, 10, 25, 50, 100, 250, 500 g in poly bottle

300 mg in poly bottle

10 mg in glass bottle