Analysis Note

ControlRIPA lysates from 3T3/A31 cells
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Application

Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH.

Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.

Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

Immunogen

6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C.

Format: Purified

Quality

Evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells.

Specificity

PLK1

Storage and Stability

Stable for 1 year at from date of receipt.

Target description

64 kDa