Amersham ECL Plex Cy3 and Cy5 labelled antibodies are part of an optimised system for quantitative analysis using fluorescent Western blotting. Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents.

Amersham Cy3 and Cy5 secondary antibodies are labelled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.

• Broad dynamic range
• High sensitivity
• Ability to detect multiple proteins on same blot (multiplexing)
• Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers
• High photostability
• Signal longevity >3 months