Application

GDF-8 Propeptide, His Tag™, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells.

Biochem/physiol Actions

ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.

General description

Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.Thies, R.S. et al. 2001. Growth Factors18, 251.Chang, H. et al. 2002. Endocrine Reviews23, 787.McPherron, A.C. et al. 1997. Nature387, 83.

Packaging

25 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)