Biochem/physiol Actions

β-Glucuronidase, a member of the 2 glycosyl hydrolase family, is involved in the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase catalyzes the complete hydrolysis of urinary conjugates. β-Glucuronidase serves as a possible candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. β-glucuronidase-responsive albumin binding prodrugs might be an effective therapeutic practice for treating solid tumors.

General description

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shibasaki, H., et al. 2001. Steroids66, 795.Graef, V., et al. 1977. Clin. Chem.23, 532.

Packaging

1 mu in Glass bottle

Physical form

A 65% saturated ammonium sulfate suspension.

Reconstitution

Following reconstitution, store in refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will liberate 1.0 µg phenolphthalein from phenolphthalein glucuronide per h at 37°C, pH 5.0.

Warning

Toxicity: Standard Handling (A)