General description

Native glutaredoxin-S₂ isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ruoppolo, M., et al. 1997. Biochemistry36, 12259.Sun, C., et al. 1997. Protein Sci.6, 383.Prinz, W.A., et al. 1997. J. Biol. Chem.272, 15661.Holmgren, A. and Alund, F. 1995 Methods Enzymol.252, 283.Hoog, J.O., et al. 1986. Gene43, 13.

Packaging

1 mg in Plastic ampoule

Physical form

Lyophilized from 0.5% NH₄HCO₃.

Reconstitution

Reconstitute in 1 ml 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 to yield a final concentration of 1 mg/ml.

Unit Definition

Units are defined using a standard HED assay.

Warning

Toxicity: Standard Handling (A)