Biochem/physiol Actions

1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5

General description

Recombinant, human GSK-3β fused at the N-terminus to a His Tag™ sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer′s disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90^rsk, c-Jun, and CREB. MW: 53,000-56,000

Recombinant, human GSK-3β fused at the N-terminus to a His Tag™ sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer's disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90^rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol.5, 275.Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 0.1 M NH₄CO₃.

Reconstitution

Reconstitute in sterile PBS to a final concentration of 1 mg/ml.

Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.

Warning

Toxicity: Standard Handling (A)