Analysis Note

Positive ControlPervanadate-treated HepG2 (included)

Application

ELISA (0.05 µg/ml)r>Immunoblotting (0.5 µg/ml)

General description

This PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

1 set in Plastic ampoule

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Reconstitution

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Warning

Toxicity: Standard Handling (A)