General description

Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.

Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810.Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.

Packaging

10 µg in Plastic ampoule

Physical form

In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.

Unit Definition

One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.

Warning

Toxicity: Standard Handling (A)