Biochem/physiol Actions

ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.r>Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.

General description

Dkk-1, His Tag™, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-dervied mouse myeloma cells.

Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Pinzone, J.J. et al. 2009. Blood113, 517.Li, J. et al. 2006. Bone36, 754.Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.Niehrs, C. 2006. Oncogene25, 7469.Verani, R. et al. 2006. J. Neurochem.101, 242.Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.Kemp, C. et al. 2005. Dev. Dyn.233, 1064.Adamska, M. et al. 2004. Dev. Biol.272, 134.Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.Mao, B. et al. 2002. Nature417, 664.Mao, B. et al. 2001. Nature411, 321.Glinka, A. et al. 1998. Nature391, 357.

Packaging

10 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Warning

Toxicity: Regulatory Review (Z)