Biochem/physiol Actions

ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

General description

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND₅₀ = 0.02-0.1 µg/ml.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Pinzone, J.J., et al. 2009. Blood113, 517.Li, J., et al. 2006. Bone36, 754.Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.Niehrs, C., 2006. Oncogene25, 7469.Verani, R., et al. 2006. J. Neurochem.101, 242.Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.Kemp, C., et al. 2005. Dev. Dyn.233, 1064.Adamska, M., et al. 2004. Dev. Biol.272, 134.Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.Mao, B., et al. 2002. Nature417, 664.Mao, B., et al. 2001. Nature411, 321.Glinka, A., et al. 1998. Nature391, 357.

Packaging

10 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Reconstitution

Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)