Biochem/physiol Actions

ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

General description

Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Li, J. et al. 2006. Bone36, 754.Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.Niehrs, C. 2006. Oncogene25, 7469.Verani, R. et al. 2006. J. Neurochem.101, 242.Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.Kemp, C. et al. 2005. Dev. Dyn.233, 1064.Adamska, M. et al. 2004. Dev. Biol.272, 134.Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.Mao, B. et al. 2002. Nature417, 664.Mao, B. et al. 2001. Nature411, 321.Krupnik, V.E., et al. 1999. Gene238, 301.

Packaging

10 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Warning

Toxicity: Standard Handling (A)