Application

Immunoblotting (1:1000)

General description

Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes G_iα-1 subunit.

Recognizes G_iα-1 subunit. Does not cross-react with G_iα-2, G_iα-3, G_oα, or G_sα subunits.

Immunogen

a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse G_iα-1, conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with G_iα-2, G_iα-3, G_oα, or G_sα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_iα-1, G_iα-2, G_iα-3, G_oα and G_sα. Variables associated with assay conditions will dictate the proper working dilution.

Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Packaging

50 µL in Plastic ampoule

Physical form

Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)