Application

Immunoblotting (1:1000)r>Neutralization Studies (see comments)

General description

Anti-G_qα- and G₁₁α-Subunits, Internal (283-300), rabbit polyclonal, recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunits. Validated for use in WB and neutralization studies.

Protein A purified rabbit polyclonal antibody. Recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunit proteins.

Recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunits. Does not cross-react with other G-proteins of the non-pertussis toxin-sensitive family.

Immunogen

a synthetic peptide (IMYSHLVDYFPEYDGPQR) corresponding to an internal amino acid sequence shared by human, mouse, and rat G_qα- and G₁₁α-subunits

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wange, R.L., et al. 1991. J. Biol. Chem. 266, 11409.Pang, I-H., and Sternweis, P.C. 1990. J. Biol. Chem. 265, 18707.Strathmann, M., et al. 1989. Proc. Natl. Acad. Sci. USA86, 7407.

The antibody recognizes both G_qα and G₁₁α since they both share the internal sequence represented by the immunizing peptide. The antibody may also be useful to block receptor G protein coupling. Does not cross-react with other G proteins of the non-pertussis toxin sensitive family. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

50 µL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)