General description

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His Tag™ sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Waltregny, D., et al. 2005. FASEB J.19, 966.Buggy, J.J., et al. 2000. Biochem J. 350, 199

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN^®-20 detergent, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.

Warning

Toxicity: Standard Handling (A)