Analysis Note

Positive Control3T3 cells, Human breast cancer tissue

Application

ELISA (see comments)r>Enzyme Immunoassay (see comments)r>Immunoblotting (1 µg/ml, colorimetric)r>Immunocytochemistry (1 µg/ml)r>Immunofluorescence (5 µg/ml)r>Immunoprecipitation (5 µg/ml)r>Paraffin Sections (see comments)

General description

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.

Recognizes the ~47 kDa Hsp47 protein.

Immunogen

Hsp47 from L6 cells

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.Nandan, D., et al. 1998. Exp. Cell Res.179, 289.Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.Saga, S., et al. 1987. J. Cell Biol.105, 517.Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)