Analysis Note

Positive ControlL929 cells, Human colon cancer tissue

Application

ELISA (see comments)r>Flow Cytometry (see comments)r>Frozen Sections (5 µg/ml) r>Immunoblotting (1 µg/ml) r>Immunocytochemistry (5 µg/ml) r>Immunoprecipitation (see comments)r>Paraffin Sections (5 µg/ml)

General description

Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.

Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).

Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.

Immunogen

Hsp70 from HeLa cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Hang, H., and Fox, M.H. 1995. Cytometry19, 119.Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.Gower, D.J., et al. 1989. J. Neurosurg.70, 605.Milarski, K., et al. 1989.J. Cell Biol.108, 413.Vass, K., et al. 1988. Acta Neuropathologica77, 128.Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following iniital thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)