Analysis Note

Positive ControlHeat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells

Application

ELISA (see comments)r>Immunoblotting (2 µg/ml, colormetric)r>Immunocytochemistry (see comments)r>Immunohistochemistry (see comments)r>Immunoprecipitation (see comments)r>Electron Microscopy (see comments)

General description

Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Rat mAb (16F1) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Electron Microscopy for the detection of Hsp90.

Protein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

Immunogen

Hsp90 isolated from therapeutic human orchiectomy specimens

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.

Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.Lanks, K.W., et al. 1982. J. Biol. Chem.257, 8604.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)