Application

ELISA (1:500-1:1000)r>Immunoblotting (1:1000-1:5000)r>Immunohistochemistry (1:100-1:500)

General description

Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.

Immunogen

a 2:1 N^α-acetyllysine-HNE fluorophore, conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.

Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µL in Glass bottle

Physical form

Serum diluted 1:1 with glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)